The present study was designed to develop a simple and validated liquid chromatographic method for the analysis metformin hydrochloride and amlodipine besylate in synthetic mixture using ultraviolet detector. Mobile phase was optimized to obtain a fast and selective separation of drug. Flow rate was o.6 ml/min, wavelength was set at 238 nm and the volume of injection was 20 μl. Mobile phase used for better separation and resolution is acetonitrile:methanol:water at the ratio of 40:15:45 v/v/v pH 3.2 was adjusted by using ortho-phosphoric acid. The retention time for metformine hydrochloride and amlodipine besylate was found to be 3.61 and 7.25 min respectively.
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